Fig 1: Levels of specific cytokines, including galectin-1, are altered in scat plasma and serum. (A) Analysis of plasma samples from scat and littermate WT mice by cytokine array reveals significant differences in four cytokines: galectin-1, amphiregulin, Axl, IGFBP-6, and no significant difference in levels of IL-1B (n = 3). (B) Quantification of the fold change of the cytokines specified in A; galectin-1: p = 0.0154, amphiregulin: p = 0.0366, Axl: p = 0.0135, IGFBP-6: p = 0.0011, IL-1B: p = 0.5825. (C) ELISA analysis of plasma and serum samples diluted 1:10 verifies significantly decreased levels of galectin-1 in scat samples compared to littermate WT (n = 15; p = 0.0018). *p < 0.05 and **p < 0.01.
Fig 2: Model of the mechanisms of anemia in the scat mouse model. Mutant RASA3 is rendered non-functional by its mislocalization to the cytosol, leading to dysregulated Ras signaling throughout erythroid differentiation. This dysregulated signaling is associated with an accumulation in the G0/G1 phase of the cell cycle in early erythroid precursors, impairing proper proliferation and differentiation and contributing to decreased red cell mass, despite decreased apoptosis in these populations. A block in terminal differentiation with an accumulation at the polychromatic/orthochromatic stages, as well as increased ROS accumulation in the reticulocyte stage further contribute to the anemia seen as a result of the loss of functional RASA3. Secreted factors, such as galectin-1, may contribute to mediating remission in the erythropoietic microenvironment while RASA3 remains mislocalized.
Supplier Page from Abcam for Mouse Galectin 1 ELISA Kit